AccuBlue?和AccuClear? 雙鏈DNA定量測(cè)定法允許純化后的雙鏈DNA樣本在較寬的范圍濃度下精確定量。不像基于光譜吸收的核酸定量方法，熒光DNA結(jié)合染料具有較高的敏感度和對(duì)雙鏈DNA的專屬選擇性，即使在有污染RNA和其他常見(jiàn)污染物，包括游離核苷酸，蛋白質(zhì)，洗滌劑和鹽存在的條件下，都可以提供準(zhǔn)確的DNA濃度。
Biotium公司提供四種雙鏈DNA定量試劑盒，分別對(duì)應(yīng)不同的儀器和樣品濃度范圍。試劑盒也針對(duì)不同的污染物有各自的耐受度（具體請(qǐng)下載相關(guān)產(chǎn)品說(shuō)明）。
要選擇合適的DNA定量檢測(cè)，請(qǐng)?jiān)敿?xì)查看下列圖表，或者點(diǎn)擊瀏覽本頁(yè)底部的相關(guān)產(chǎn)品詳細(xì)信息。
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) | Ex/Em (nm) | Features |
|---|---|---|---|
| AccuBlue? Broad Range | 2-2000 ng | 350/460 | For quantitation of high concentration DNA in a broad linear range. Assay can be extended to 4000 ng with minor loss of linearity.? Compatible with?AccuLite? 350 Mini-Fluorometer?or fluorescence microplate reader. |
| AccuBlue? High Sensitivity | 0.2-100 ng | 485/530 | Compatible with?AccuLite? 470 Mini-Fluorometer, Qubit? fluorometers, or fluorescence microplate reader. |
| AccuClear? Ultra High Sensitivity | 30 pg-250 ng | 468/507 | For quantitation of low concentration DNA, this kit offers great sensitivity and a wide linear range.? Compatible with?AccuLite? 470 Mini-Fluorometer?or fluorescence microplate reader. |
| NEW!AccuBlue? NextGen | 1 pg*-3 ng | 468/507 | Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.? Compatible with?AccuLite? 470 Mini-Fluorometer?or fluorescence microplate reader. |
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圖2.使用AccuBlue和AccuClear經(jīng)四個(gè)簡(jiǎn)單的步驟便可定量測(cè)定大量樣品。 |
圖3.選擇性測(cè)定雙鏈DNA。小牛胸腺雙鏈DNA，小鼠肝臟RNA或病毒的M13mp18單鏈DNA檢測(cè)的AccuBlue高靈敏度雙鏈DNA定量試劑盒。 |
NEW! AccuBlue? NextGen
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圖4.使用AccuBlue NextGen Kit的小牛胸腺DNA的標(biāo)準(zhǔn)曲線測(cè)定，微板酶標(biāo)儀讀取 (Ex/Em 460/507).內(nèi)圖顯示了檢測(cè)的下限。 |
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AccuClear? Ultra High Sensitivity
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圖5.使用AccuClear Ultra High Sensitivity Kit 的小牛胸腺DNA的標(biāo)準(zhǔn)曲線測(cè)定，微板酶標(biāo)儀讀取(Ex/Em 460/507).內(nèi)圖顯示了檢測(cè)的下限。 |
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AccuBlue? High Sensitivity
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圖6.使用 AccuBlue High Sensitivity Kit 的小牛胸腺DNA的標(biāo)準(zhǔn)曲線測(cè)定，微板酶標(biāo)儀讀?。?Ex/Em 485/530).內(nèi)圖顯示了檢測(cè)的下限。 |
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AccuBlue? Broad Range
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圖7.使用 AccuBlue 或者 Quant-iT Broad Range assay kits 測(cè)定兩倍稀釋的小牛胸腺DNA，AccuBlue比Quant-iT 有更好的線性相關(guān)性和更寬的動(dòng)態(tài)范圍。 |
訂貨信息：
| 編號(hào) | 產(chǎn)品名稱/規(guī)格 | 價(jià)格（rmb） |
生產(chǎn)商 |
| 31007 | AccuBlue Broad Range dsDNA Quantitation Kit 1KIT |
￥3304 | Biotium Inc. |
| 31007-T | AccuBlue Broad Range dsDNA Quantitation Kit with DNA Standard, trial size (200 assays) 1KIT |
￥742 | Biotium Inc. |
| 31009 | AccuBlue Broad Range dsDNA Quantitation Solution 1KIT |
￥2212 | Biotium Inc. |
| 31009-T | AccuBlue Broad Range dsDNA Quantitation Solution, trial size 1KIT |
￥588 | Biotium Inc. |
| 31007C | AccuBlue Broad Range dsDNA Quantitation Standards, set of nine, 0.5 mL each 1KIT |
￥1106 | Biotium Inc. |
| 31006 | AccuBlue High Sensitivity dsDNA Quantitation Kit 1KIT |
￥3304 | Biotium Inc. |
| 31006-T | AccuBlue High Sensitivity dsDNA Quantitation Kit with DNA Standard, trial size (200 assays) 1KIT |
￥742 | Biotium Inc. |
| 31008 | AccuBlue High Sensitivity dsDNA Quantitation Solution 1KIT |
￥2212 | Biotium Inc. |
| 31008-T | AccuBlue High Sensitivity dsDNA Quantitation Solution, trial size 1KIT |
￥588 | Biotium Inc. |
| 31006C | AccuBlue High Sensitivity dsDNA Standards, set of eight, 0.5 mL each 1KIT |
￥1106 | Biotium Inc. |
| 31060 | AccuBlue NextGen dsDNA Quantitation Kit (1000 assays) 1kit |
￥5152 | Biotium Inc. |
| 31060-T | AccuBlue NextGen dsDNA Quantitation Kit, Trial Size (200 assays) 1kit |
￥1176 | Biotium Inc. |
| 31061 | AccuBlue NextGen dsDNA Quantitation Solution (1000 assays) 1kit |
￥3388 | Biotium Inc. |
| 31061-T | AccuBlue NextGen dsDNA Quantitation Solution, Trial Size (200 assays) 1kit |
￥812 | Biotium Inc. |
| 31029 | AccuClear Ultra High Sensitivity dsDNA Quantitation Assay kit with 1 DNA Standard (2000 assays) 1kit |
￥5446 | Biotium Inc. |
| 31028 | AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards (1000 assays) 1kit |
￥3682 | Biotium Inc. |
| 31028-T | AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standard, trial size (200 assays) 1kit |
￥882 | Biotium Inc. |
| 31027 | AccuClear Ultra High Sensitivity dsDNA Quantitation Solution (1000 assays) 1kit |
￥2352 | Biotium Inc. |
| 31027-T | AccuClear Ultra High Sensitivity dsDNA Quantitation Solution, Trial Size (200 assays) 1kit |
￥742 | Biotium Inc. |